Sharp Image of a Cell made by Glowing DNA 1

Very sharp image of a cell, made with glowing DNA, captured using a new technique called Exchange-Paint.   Image © Maier Avendano/Wyss Institute

This ultrasharp image uses a new method to simultaneously resolve microtubules (green), mitochondria (purple), Golgi apparatus (red), and peroxisomes (yellow) from a single human cell. The scale bar is 5 microns.

It is the first time to take images less than 10nm apart and this lead us in new ways to to diagnose disease, track its progress.

Such images could shed light on complex cellular pathways and potentially lead to new ways to diagnose disease, track its prognosis, or monitor the effectiveness of therapies at a cellular level.

Cells often employ dozens or even hundreds of different proteins and RNA molecules to get a complex job done. As a result, cellular job sites can resemble a busy construction site, with many different types of these tiny cellular workers coming and going. Today’s methods typically only spot at most three or four types of these tiny workers simultaneously. But to truly understand complex cellular functions, it’s important to be able to visualize most or all of those workers at once, said Peng Yin, Ph.D., a Core Faculty member at the Wyss Institute and Assistant Professor of Systems Biology at Harvard Medical School.

“If you can see only a few things at a time, you are missing the big picture,” Yin said.

Sharp Image of a Cell made by Glowing DNA 2

Ten-color super-resolution images (artificially arranged into one composite) of synthetic DNA nanostructures displaying patterns that resemble the numerals “0” through “9”, produced by Exchange-PAINT using only a single dye and a single laser source. Credit: Johannes B. Woehrstein/Wyss Institute”.


via wired

source Harvard